Wound heal­­ing ef­fects after application of polyunsaturated fatty acids in rat

Authors: A. Hokynková 1;  Z. Wilhelm 2;  M. Nováková 2;  P. Babula 2;  T. Stračina 2;  H. Paulová 3;  M. Hlaváčová 3;  M. Sedláčková 4
Authors‘ workplace: Klinika popálenin a plastické chirurgie LF MU a FN Brno 1;  Ústav fyziologie, LF MU a FN Brno 2;  Ústav biochemie, LF MU a FN Brno 3;  Ústav histologie a embryologie, LF MU a FN Brno 4
Published in: Cesk Slov Neurol N 2018; 81(Suplementum 1): 29-31
Category: Original Paper
doi: https://doi.org/10.14735/amcsnn2018S29



Although mechanisms of polyunsaturated fatty acids influence on skin wound heal­­ing have not been ful­ly elucidated yet, it is undisputable that nutritional state af­fects it profoundly.


The study was focused on speed and quality of wound heal­­ing influenced by short-last­­ing oral administration of omega-3 and omega-6 polyunsaturated fatty acids.

Materials and methods: 

Young Wistar rats received by orogastric tube either 20% fat emulsion (experimental groups E and F dif­fered by ratio between omega-3 : omega-6 : omega-9 polyunsaturated fatty acids) or water (control group C). After 7 days, skin wound on back was performed and emulsion or water application continued for another week. Blood and tis­sue samples were obtained dur­­ing experiment termination. Plasmatic levels of fatty acids, 4-hydroxy-2-nonenal and oxidative stress both in plasma and in wound tis­sue were examined. Speed and quality of wound heal­­ing were as­ses­sed by digital planimetry and histological examination. Results were statistical­ly evaluated by non-parametric tests.


Significant changes of fatty acids plasmatic levels were observed in animals in experimental groups as well as better ability of their plasma and tis­sues to cope with oxidative stres­s.


Short-last­­ing administration of certain ratio of polyunsaturated fatty acids positively af­fects skin wound healing.

Key words:

polyunsaturated fatty acids – wound healing – anti-oxidative capacity – 4-HNE – 4-hydroxy-2-nonenal

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manu­script met the ICMJE “uniform requirements” for biomedical papers.


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